A Stability Indicating Hplc Method for the Determination of Mepivacaine Hydrochloride

Stability indicating HPLC method has been developed for specific determination of Mepivacaine hydrochloride during degradation under acid, alkali, thermal and oxidative stress conditions. Methanol: Orthophosphoric acid solution (2.25 gm/L) adjusted to pH 7.6 with strong sodium hydroxide solution (75:25), was used as the mobile phase, at a flow rate of 1 ml/min. A Gracesmart C18 column (5μ), 250 mm, 4.6 mm i.d. was used as stationary phase. Detection was affected spectrophotometrically at 215 nm. The retention time was approximately 4.7 min. Linearity range was 20-100 μg/ml. The minimum detection and Quantitation limits were 1.146 and 3.465 μg/ml respectively. Proposed method was validated for precision, accuracy, ruggedness, robustness and specificity.